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The cytotoxic effect was tested through the colorimetric assay employing 3′-[1-[(phenylamino) -carbonyl]−3,4-tetrazolium]-bis(4‑methoxy-6-nitro) benzene-sulfonic acid hydrate (XTT) by reading the absorbance at 490 nm after 3 h of incubation post treatment [28]. The absorbance is proportional to the metabolic rate of viable (live) cells.

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Another popular method is inductively coupled plasma-mass spectrometry (ICP-MS), which uses a high-temperature plasma to ionize sample compounds and measure their mass-to-charge ratio. ICP-MS is highly sensitive and can detect sulphate at extremely low levels, making it a powerful tool for determining trace amounts of sulphate in TiO2 ICP-MS is highly sensitive and can detect sulphate at extremely low levels, making it a powerful tool for determining trace amounts of sulphate in TiO2 ICP-MS is highly sensitive and can detect sulphate at extremely low levels, making it a powerful tool for determining trace amounts of sulphate in TiO2 ICP-MS is highly sensitive and can detect sulphate at extremely low levels, making it a powerful tool for determining trace amounts of sulphate in TiO2determination of sulphate as tio2 manufacturers.

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